The aim of the present work is to generate at laboratory scale a biomaterial developed by in-vitro technology with similar characteristics to genuine leather. The generation of an in-vitro tissue frombovine fibroblasts is carried out using a scaffold to generate a three-dimensional extra-cellular matrix (3D ECM). The development of a biopelt is by an in-vitro production at lab scale in a bioreactor withspecific culturing conditions.1 New methodologies to stabilize the biomaterial generated were investigated. In particular, the biomaterial was stabilized by immersion in solutions of vegetable tanninsand cross-linking agents in an aqueous-solvent medium.