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Extraction and purification of hydrolytic enzymes from activated sludge
Nabarlatz, Debora; Stüber, Frank Erich; Font Capafons, Josep; Fortuny Sanromá, Agustín; Fabregat Llangotera, Azael; Bengoa, Christophe José
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química
A major proportion of the organic matter contained by domestic wastewater is mainlyformed by lipids, proteins and carbohydrates. In order to degrade this organic matter,microorganisms produce hydrolytic enzymes like proteases, lipases and other enzymes thatcannot be produced by standard cultivation techniques, which makes its recovery of interest. Inthe present study protease and lipase were extracted by using magnetic stirring and ultrasounddisintegration combined with different additives. It was observed that the concentration ofTriton X100 has a great influence in the extraction of protease, but it has no effect in theextraction of lipase. Samples obtained after ultrasound disintegration with 0% and 2% TritonX100 were further purified by precipitation with ammonium sulphate and dialysis. Thesesamples were frozen and lyophilized in order to recover them in powder form. The optimalprocess for the recovery of lipase was a combination of ultrasound treatment using 0% TX100,followed by dialysis and lyophilization. This process allowed recovering around 23 lipaseunits/g solid.
2012-05-10
Àrees temàtiques de la UPC::Desenvolupament humà i sostenible::Enginyeria ambiental::Tractament dels residus
Àrees temàtiques de la UPC::Enginyeria química::Química del medi ambient::Química de l'aigua
Sludge management series
Aigües residuals -- Depuració -- Eliminació de compostos orgànics
Aigües residuals -- Depuració -- Procés de fangs activats
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