The goal of the Soil Moisture and Ocean Salinity mission over land is to infer surface soil moisture from multiangular L-band radiometric measurements. As the canopy affects the microwave emission of land, it is necessary to characterize different vegetation layers. This paper presents the Reference Pixel L-Band Experiment (REFLEX), carried out in June-July 2003 at the Vale/spl grave/ncia Anchor Station, Spain, to study the effects of grapevines on the soil emission and on the soil moisture retrieval. A wide range of soil moisture (SM), from saturated to completely dry soil, was measured with the Universitat Polite/spl grave/cnica de Catalunya's L-band Automatic Radiometer (LAURA). Concurrently with the radiometric measurements, the gravimetric soil moisture, temperature, and roughness were measured, and the vines were fully characterized. The opacity and albedo of the vineyard have been estimated and found to be independent on the polarization. The /spl tau/--/spl omega/ model has been used to retrieve the SM and the vegetation parameters, obtaining a good accuracy for incidence angles up to 55/spl deg/. Algorithms with a three-parameter optimization (SM, albedo albedo, and opacity) exhibit a better performance than those with one-parameter optimization (SM).