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Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development
Calvo-Garrido, Javier; Carilla-Latorre, Sergio; Lázaro Diégez, Francisco; Egea Guri, Gustavo
Universitat de Barcelona
Vacuole membrane protein 1 (Vmp1) is membrane protein of unknown molecular function that has been associated with pancreatitis and cancer. The social amoeba Dictyostelium discoideum has a vmp1-related gene that we identified previously in a functional genomic study. Loss-of-function of this gene leads to a severe phenotype that compromises Dictyostelium growth and development. The expression of mammalian Vmp1 in a vmp1 Dictyostelium mutant complemented the phenotype, suggesting a functional conservation of the protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to survive under hypoosmotic conditions, is not functional in the mutant. The structure of the Golgi apparatus, the function of the endocytic pathway and conventional protein secretion are also affected in these cells. Transmission electron microscopy of vmp1 cells showed the accumulation of autophagic features that suggests a role of Vmp1 in macroautophagy. In addition to these defects observed at the vegetative stage, the onset of multicellular development and early developmental gene expression are also compromised.
2012-05-02
Proteïnes de membrana
Citologia
Citogenètica
Reticle endoplasmàtic
Membrane proteins
Cytology
Cytogenetics
Endoplasmic reticulum
cc-by-nc-sa (c) Calvo-Garrido et al., 2008
http://creativecommons.org/licenses/by-nc-sa/3.0/
Article
Article - Published version
American Society for Cell Biology
         

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