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Diversity of Vibrionaceae bacteria isolated from the haemolymph of the spider crab Maja brachydactyla
Gomez-Gil, Bruno; Roque, A.; Lacuesta, B.; Rotllant Estelrich, Guiomar
IRTA. Recerca i Tecnologia Agroalimentàries
Aims: The aim of this study was to characterise and identify vibrios isolated from the haemolymph of apparently healthy adult spider crabs (Maja brachydactyla) wild-caught in the Spanish localities of Galician coast and in the Canary Islands and also from captive animals held at IRTA’s facilities in the Ebro Delta of Catalonia, north-west Spanish Mediterranean coast. Methods and Results: A total of 277 bacterial isolates were obtained, and of these, 171 were characterised with rep-PCR, resulting electrophoretic bands were analysed and clusters formed. Identification of representative strains of each cluster was made by sequencing the 16S rRNA. Samples from animals caught in Galicia and captive at IRTA (around 15–18 C) rendered mostly species belonging to the Splendidus clade (72Æ2 and 76Æ6% respectively), commonly found in cold waters (below 20 C). Higher species diversity was found in the haemolymph of the captive animals. In the warmer Canary Islands waters (around 21 C), the diversity of vibrios is dominated by three clades, Harveyi (Vibrio core group, 39Æ3%), Orientalis (23Æ2%) and Splendidus (21Æ4%) with a species diversity that equals that of the colder captive animals. Conclusions: Differences in the vibrios populations were found in the haemolymph extracted from animals collected from the three localities. Potential new species were found, and their description is under way. Significance and Impact of Study: As with other invertebrates, spider crabs also contain a diverse population of vibrios. These findings should help researchers to diagnose when a crab is infected.
2010
63 - Agricultura. Silvicultura. Zootècnia. Caça. Pesca
Crustacis -- Conservació
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/ 
28 p.
Article
Wiley-Blackwell
Reproducció del document publicat a:http://dx.doi.org/10.1111/j.1365-2672.2010.04718.x
         

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Andrée, Karl; Boglino, Anais; Carazo, I.; Carbó, R.; Carnicer, O.; Carrasco, N.; Chauvigné, F.; Celades, J.L.; Cerdà Luque, Joan; Damaso Cid, Esther; Darias, M.J.; De la Iglesia, Pablo; Diogène Fadini, Jorge; Duncan, N.J.; Estévez García, Alicia; Fernandez, I.; Fernández, Margarita; Fernández Tejedor, M.; Furones, Dolors; Gairin Deulofeu, Joan Ignasi; Gimenez, G.; Gisbert Casas, Enric; Guerao Serra, Guillermo; Lacuesta, B.; Lopez-Joven, C.; Macià, G.; Rodgers, C.; Roque, A.; Rotllant Estelrich, Guiomar; Sagristà, N.; Sastre, M.; Simeó, C.; Skalli, A.; Vallés, R.; Verdura, S.
 

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