http://www.recercat.cat:80/handle/2072/201103 Wed, 22 May 2013 13:49:38 GMT 2013-05-22T13:49:38Z http://www.recercat.cat:80/bitstream/id/54855/ http://www.recercat.cat:80/handle/2072/201103 http://www.recercat.cat:80/handle/2072/202051 Isotope Ratio - Mass Spectrometry Marimon, Rosa Maria; Perona, Joaquim; Teixidor, Pilar This article summarizes the configurations involving isotope ratio mass spectrometry (IRMS) technology available at the CCiTUB and the wide range of possible applications. Some examples of these applications are shown. http://www.recercat.cat:80/handle/2072/202051 http://www.recercat.cat:80/handle/2072/202052 Photoelectron spectroscopy for surface analysis: X-ray and UV excitation Calvo-Barrio, Lorenzo; Vargas, Gardenia This article summarizes the basic principles of photoelectron spectroscopy for surface analysis, with examples of applications in material science that illustrate the capabilities of the related techniques. http://www.recercat.cat:80/handle/2072/202052 http://www.recercat.cat:80/handle/2072/202053 Electron Energy Loss Spectroscopy: physical principles, state of the art and applications to the nanosciences Estradé, Sònia In the present work we review the way in which the electron-matter interaction allows us to perform electron energy loss spectroscopy (EELS), as well as the latest developments in the technique and some of the most relevant results of EELS as a characterization tool in nanoscience and nanotechnology. http://www.recercat.cat:80/handle/2072/202053 http://www.recercat.cat:80/handle/2072/202048 Basics of mass spectrometry Berdié Rabanaque, Lourdes; Casals i Ribes, Isidre; Fernández Vidal, Irene; Jáuregui Pallarés, Olga; Marimon Corbella, Rosa Maria; Perona Moreno, Joaquim; Teixidor Casamitjana, Pilar This article summarizes the basic principles of mass spectrometry instrumentation with special emphasis in sample introduction methods, ionization techniques and mass analyzers used in the different mass spectrometrytechniques.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/202048 http://www.recercat.cat:80/handle/2072/202049 Gas Chromatography - Mass Spectrometry Berdié, Lourdes; Fernández, Irene; Teixidor, Pilar This article briefly describes the possible configurations of the gaschromatography- mass spectrometry (GC/MS) technology available at the CCiTUB. Some developed examples of different applications are shown. http://www.recercat.cat:80/handle/2072/202049 http://www.recercat.cat:80/handle/2072/202050 Liquid Chromatography - Mass Spectrometry Jáuregui, Olga; Casals, Isidre; Fernández, Irene In this article, selected examples of applications of liquid chromatography coupled to mass spectrometry are given. The examples include the analysis of i) impurities in manufactured, pharmaceutical or synthesis products, ii) polyphenols in natural products, and iii) phytohormones in plant extracts. Finally, examples of applications of molecular characterization via flow injection analysis by electron spray ionization mass spectrometry (ESI-MS) are also given. http://www.recercat.cat:80/handle/2072/202050 http://www.recercat.cat:80/handle/2072/202047 Nuclear magnetic resonance Feliz Rodenas, Miguel; Molins i Montserrat, Mª Antònia; Gairí Tahull, Margarida; Muñoz-Torrero López-Ibarra, Victoria; Gonzalez, Teresa This article reviews the principles and methods of nuclear magnetic resonance spectroscopy, and gives examples of applications carried out at ourFacility, which illustrate the capabilities of the technique.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/202047 http://www.recercat.cat:80/handle/2072/202043 Electron probe microanalysis: principles and applications Llovet, Xavier This article summarizes the basic principles of electron probe microanalysis, with examples of applications in materials science and geology that illustrate the capabilities of the technique. http://www.recercat.cat:80/handle/2072/202043 http://www.recercat.cat:80/handle/2072/202044 Advanced applications of Scanning Electron Microscopy in Geology García-Veigas, Javier; Prats, Eva; Domínguez, Anna; Villuendas, Aranzazu Nowadays Scanning Electron Microscopy (SEM) is a basic and fundamental tool in the study of geologic samples. The collision of a highlyaccelerated electron beam with the atoms of a solid sample results in theproduction of several radiation types than can be detected and analysed byspecific detectors, providing information of the chemistry and crystallography ofthe studied material. From this point of view, the chamber of a SEM can beconsidered as a laboratory where different experiments can be carried out. Theapplication of SEM to geology, especially in the fields of mineralogy andpetrology has been summarised by Reed (1996).The aim of this paper is to showsome recent applications in the characterization of geologic materials. http://www.recercat.cat:80/handle/2072/202044 http://www.recercat.cat:80/handle/2072/202045 Advanced Optical Microscopies for Materials: New trends Diaz-Marcos, Jordi; Pinna, Francesca; Oncins, Gerard This article summarizes the new trends of Optical Microscopy applied to Materials, with examples of applications that illustrate the capabilities of thetechnique. http://www.recercat.cat:80/handle/2072/202045 http://www.recercat.cat:80/handle/2072/202046 Applications of Fourier transform infrared spectroscopy Ferrer i Felis, Núria This article summarizes the basic principles of Fourier Transform Infrared Spectroscopy, with examples of methodologies and applications to different field sciences.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/202046 http://www.recercat.cat:80/handle/2072/202042 Precession Electron Diffraction in the Transmission Electron Microscope: electron crystallography and orientational mapping Portillo, Joaquim Precession electron diffraction (PED) is a hollow cone non-stationary illumination technique for electron diffraction pattern collection under quasikinematicalconditions (as in X-ray Diffraction), which enables “ab-initio” solving of crystalline structures of nanocrystals. The PED technique is recently used in TEMinstruments of voltages 100 to 300 kV to turn them into true electron iffractometers, thus enabling electron crystallography. The PED technique, when combined with fast electron diffraction acquisition and pattern matching software techniques, may also be used for the high magnification ultra-fast mapping of variable crystal orientations and phases, similarly to what is achieved with the Electron Backscatter Diffraction (EBSD) technique in Scanning ElectronMicroscopes (SEM) at lower magnifications and longer acquisition times. http://www.recercat.cat:80/handle/2072/202042 http://www.recercat.cat:80/handle/2072/202041 Raman spectroscopy as a powerful analytical tool: probing the structure of matter Jawhari, Tariq Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/202041 http://www.recercat.cat:80/handle/2072/201906 Genomics: more than genes Seminago, Ramon; Castaño, Esther; Amador, Amaya The fundamentals of Real-time Polymerase Chain Reaction,Automated capillary electrophoresis -Sanger sequencing and Fragmentanalysis- and "Next-generation" sequencing are reviewed. An overview ofapplications is presented using our own examples carried out in our facility. http://www.recercat.cat:80/handle/2072/201906 http://www.recercat.cat:80/handle/2072/201907 "Next-generation" Sequencing (NGS): The new genomic revolution Fusté, Berta In the past 5 years "Next-generation" Sequencing (NGS) technologies have transformed genomics by delivering fast, inexpensive and accurate genomeinformation changing the way we think about scientific approaches in basic,applied and clinical research. The inexpensive production of large volumes ofsequence data is the main advantage over the automated Sanger method,making this new technology useful for many applications. In this chapter, a brieftechnical review of NGS technologies is given, along with the keys to NGSsuccess and a broad range of applications for NGS technologies. http://www.recercat.cat:80/handle/2072/201907 http://www.recercat.cat:80/handle/2072/201908 Analyzing Peptides and Proteins by Mass Spectrometry: Principles and applications in Proteomics Fidalgo, Maria José; Olmedo, Eva; Fernández, Irene; Estanyol, Josep Maria The study of proteins has been a key element in biomedicine and biotechnology because of their important role in cell functions or enzymatic activity. Cells are the basic unit of living organisms, which are governed by a vast range of chemical reactions. These chemical reactions must be highly regulatedin order to achieve homeostasis. Proteins are polymeric molecules that havetaken on the evolutionary process the role, along with other factors, of controlthese chemical reactions. Learning how proteins interact and control their up anddown regulations can teach us how living cells regulate their functions, as well asthe cause of certain anomalies that occur in different diseases where proteins areinvolved. Mass spectrometry (MS) is an analytical widely used technique to studythe protein content inside the cells as a biomarker point, which describesdysfunctions in diseases and increases knowledge of how proteins are working.All the methodologies involved in these descriptions are integrated in the fieldcalled Proteomics. http://www.recercat.cat:80/handle/2072/201908 http://www.recercat.cat:80/handle/2072/201909 Flow cytometry in the analysis of cellular populations Castaño, Esther; Comas, Jaume Flow cytometry has become a valuable tool in cell biology. By analyzing large number of cells individually using light-scatter and fluorescence measurements, this technique reveals both cellular characteristics and the levels of cellular components. Flow cytometry has been developed to rapidly enumerate cells and to distinguish among different cell stages and structures using multiple staining. In addition to high-speed multiparametric data acquisition, analysis and cell sorting, which allow other characteristics of individual cells to be studied, have increased the interest of researchers in this technique. This chapter gives an overview of the principles of flow cytometry and examples of the application ofthe technique. http://www.recercat.cat:80/handle/2072/201909 http://www.recercat.cat:80/handle/2072/201905 Bioinformatics: making large-scale science happen Beltran, Sergi Bionformatics is a rapidly evolving research field dedicated toanalyzing and managing biological data with computational resources. This paperaims to overview some of the processes and applications currently implementedat CCiT-UB¿s Bioinformatics Unit, focusing mainly on the areas of Genomics,Transcriptomics and Proteomics. http://www.recercat.cat:80/handle/2072/201905 http://www.recercat.cat:80/handle/2072/201904 Advanced Light Microscopy Techniques Bosch, Manel; Torrejón-Escribano, Benjamín; Bosch, Anna; Calvo, Maria This article summarizes the basic principles of light microscopy, with examples of applications in biomedicine that illustrate the capabilities of thetechnique. http://www.recercat.cat:80/handle/2072/201904 http://www.recercat.cat:80/handle/2072/201902 Transmission Electron Microscopy in Cell Biology: sample preparation techniques and image information Cortadellas, Núria; Garcia, Almudena; Fernández, Eva Transmission electron microscopy is a proven technique in the field of cell biology and a very useful tool in biomedical research. Innovation and improvements in equipment together with the introduction of new technology have allowed us to improve our knowledge of biological tissues, to visualizestructures better and both to identify and to locate molecules. Of all the types ofmicroscopy exploited to date, electron microscopy is the one with the mostadvantageous resolution limit and therefore it is a very efficient technique fordeciphering the cell architecture and relating it to function. This chapter aims toprovide an overview of the most important techniques that we can apply to abiological sample, tissue or cells, to observe it with an electron microscope, fromthe most conventional to the latest generation. Processes and concepts aredefined, and the advantages and disadvantages of each technique are assessedalong with the image and information that we can obtain by using each one ofthem. http://www.recercat.cat:80/handle/2072/201902 http://www.recercat.cat:80/handle/2072/201899 Liquid and solid scintillation: principles and applications Benito González, Carmen; Jiménez Guerrero, Andrés; Sánchez García, Cristina; Navarro i Sabaté, Àurea Scintillation counting is one of the most important developments in the application of radioisotopes to procedures needed by scientists, physicians, engineers, and technicians from many diverse discipline for the detection and quantitative measurement of radioactivity. In fact, Scintillation is the most sensitive and versatile technique for the detection and quantification ofradioactivity. Particularly, Solid and Liquid scintillation measurement are,nowadays, standard laboratory methods in the life-sciences for measuringradiation from gamma- and beta-emitting nuclides, respectively. Thismethodology is used routinely in the vast majority of diagnostic and/or researchlaboratories from those of biochemistry and biology to clinical departments.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201899 http://www.recercat.cat:80/handle/2072/201900 X-Ray systems: radiation protection program in basic research applications Navarro i Sabaté, Àurea; Sánchez García, Cristina Although the radiation doses involved in basic research radiology are relatively small, the increasing number of radiological procedures makes risks becoming increasingly high. Quality control techniques in radiological practice have to ensure an adequate system of protection for people exposed to radiation. These techniques belong to a quality assurance program for X-ray machines and are designed to correct problems related to equipment and radiological practices, to obtain radiological images of high quality and to reduce the unnecessary exposures.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201900 http://www.recercat.cat:80/handle/2072/201901 Crystal engineering studies: polymorphs and co-crystals Prohens, Rafel; Puigjaner Vallet, Ma. Cristina We review the key topics of one of the areas with the biggest impact of the last years in the chemical and pharmaceutical industry that is Crystal Engineering. The relevance of polymorphs and co-crystals from different points of view is been highlighted and broadly illustrated by means of several recent examples of studies carried out in this field. In addition, the most suitableinstrumental techniques and the intellectual property implications are reviewed.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201901 http://www.recercat.cat:80/handle/2072/201903 Biomedical and Biological Applications of Scanning Electron Microscopy Cortadellas, Núria; Fernández, Eva; Garcia, Almudena This article summarizes the basic principles of scanning electron microscopy and the capabilities of the technique with different examples ofapplications in biomedical and biological research. http://www.recercat.cat:80/handle/2072/201903 http://www.recercat.cat:80/handle/2072/201897 Overview of molecular interactions using Biacore Taulés i Marín, Marta; Comas Riu, Jaume F. Surface Plasmon Resonance (SPR) technology is a powerful tool for studying a wide range of different putative interactions. This kind of optical biosensors allow to obtain (in real time and without labelling)quantitative and qualitative information about the kinetics of the surfacebindingprocess. The most critical points to keep in mind when using the technique are presented, as well as practical examples of applications.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201897 http://www.recercat.cat:80/handle/2072/201898 Radiation protection education and training in several ionising radiation applications Rafecas Jorba, Immaculada Ionising radiation (IR) applications are quiet common among several areas of knowledge, medicine or industry. Medical X-rays, Nuclear Medicine, Xrays used in non-destructive testing or applications in research are a few examples. These radiations originate from radioactive materials or radiation emitting devices. Radiation Protection education and training (E&T) is of paramount importance to work safely in areas that imply the use of IR. TheTechnical Unit for Radiation Protection at the University of Barcelona has anextensive expertise in basic, initial and refresher training, in general or specificareas, as well as in courses validated by the Spanish Nuclear Safety Council orto satisfy specific needs with bespoke courses. These specific customer needsare evaluated and on-site courses can also be carried out.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201898 http://www.recercat.cat:80/handle/2072/201896 3D solutions in transmission electron (Cryo) -microscopy in biology Delgado, Lídia; Martínez, Gemma; Muela, Yolanda; Hernández, Nieves; López-Iglesias, Carmen One of the main problems in transmission electron microscopy in thebiological field is the tri-dimensionality. This article explains the technicalprocedures and requirements to prepare biological specimens preserving themclosest to their native state to perform 3D reconstruction of the macromolecularcomplexes and cellular structures in their natural environment.; Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166 http://www.recercat.cat:80/handle/2072/201896