http://www.recercat.cat:80/handle/2072/13340 Sat, 25 May 2013 02:12:03 GMT 2013-05-25T02:12:03Z http://www.recercat.cat:80/bitstream/id/34951/ http://www.recercat.cat:80/handle/2072/13340 http://www.recercat.cat:80/handle/2072/179102 Evaluation of detection methods for Virus, Viroids and Phytoplasmas affecting pear and apple Laviña Gomila, Amparo; Sabaté, J.; Batlle i Durany, Assumpció The RT-PCR technique for the detection of apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple mosaic virus (ApMV) and pear blister canker viroid (PBCV) was evaluated for health control of fruit plants from nurseries. The technique was evaluated in purified RNA and crude extracts and also in phloem collected in autumn and from young spring shoots. The results obtained for phytoplasma detection with ribosomal and non-ribosomal primers are also presented. Fri, 01 Jan 2010 00:00:00 GMT http://www.recercat.cat:80/handle/2072/179102 2010-01-01T00:00:00Z http://www.recercat.cat:80/handle/2072/179101 Evaluation of susceptibility of pear and plum varieties and rootstocks to Ca. P. pyri and Ca. P. prunorum using Real-Time PCR Torres, E.; Laviña Gomila, Amparo; Sabaté, J.; Bech, J.; Batlle i Durany, Assumpció Real-time PCR was used to quantify phytoplasma concentration in fifty inoculated trees from five Prunus rootstocks and in forty-eight symptomatic pear and Japanese plum trees from orchards. Seasonal fluctuation of Ca. P. prunorum in different Prunus rootstocks, over three years, showed that the highest percentage detected by nested-PCR was in the ‘Garnem’ rootstock on nearly all sampling dates. Intra-varietal differences were also observed. Phytoplasma titer could be estimated by real time PCR in some trees of the rootstocks ‘Garnem’, ‘Barrier’, ‘GF-677’ and ‘Marianna’, and ranged from 4.7x105 to 3.18x109 phytoplasmas per gram of tissue. Quantification by real-time PCR was not possible in the ‘Cadaman’ trees analyzed, probably due to a lower phytoplasma titer in this variety. Samples from infected trees from commercial plots had different phytoplasma concentration and detection percentage depending on the variety, both being lower in ‘Fortune’ and ‘606’ Japanese plum and in ‘Blanquilla’ pear trees. Fri, 01 Jan 2010 00:00:00 GMT http://www.recercat.cat:80/handle/2072/179101 2010-01-01T00:00:00Z http://www.recercat.cat:80/handle/2072/171437 Efectividad de nuevas estrategias de control de Botrytis cinerea en viñedos de agricultura ecológica de la zona de Lleida Calvo Garrido, Carlos; Elmer, PAG; Usall i Rodié, Josep; Viñas Almenar, Immaculada; Teixidó Espasa, Neus Fri, 01 Jan 2010 00:00:00 GMT http://www.recercat.cat:80/handle/2072/171437 2010-01-01T00:00:00Z http://www.recercat.cat:80/handle/2072/13365 Mycorrhizal inoculation of grapevines in replant soils: improved field application and plant performance Nogales García, Amaia; Camprubí Nieto, Amèlia; Estaun, Victoria; Calvet Pinós, Mª Cinta Tue, 01 Jan 2008 00:00:00 GMT http://www.recercat.cat:80/handle/2072/13365 2008-01-01T00:00:00Z http://www.recercat.cat:80/handle/2072/13364 Efficacy and persistance of introduced AM fungi in rehabilitation processes Estaun, Victoria; Busquets Torras, Marta; Calvet Pinós, Mª Cinta; Camprubí Nieto, Amèlia Tue, 01 Jan 2008 00:00:00 GMT http://www.recercat.cat:80/handle/2072/13364 2008-01-01T00:00:00Z