http://hdl.handle.net/2072/4698 http://www.recercat.net/retrieve/8636 http://hdl.handle.net/2072/4698 Search the Channel s http://www.recercat.net/simple-search http://hdl.handle.net/2072/39527 title: Preliminary comparison of nutritional status of wild and culture broodstok of Senegal sole authors: Norambuena Filcun, Fernando; Estévez García, Alicia; Andrés Roig, Mireia; Carazo Ortega, Ignacio; Duncan, Neil <br> http://hdl.handle.net/2072/39526 title: Composición en ácidos grasos de huevos y larvas de dentón (Dentex dentex L.) de hasta 40 días en condiciones de cultivo authors: Giménez Papiol, Gemma; Estévez García, Alicia; Henderson, R. James; Bell, J. Gordon <br>abstract: Total lipid content and fatty acid (FA) composition of common dentex eggs spawned at different times and larvae reared under different culture conditions until 40 days post hatch (dph) were analysed in order to get a general pattern of lipid composition during larval development. Results were grouped according to the developmental stage of the larvae instead of age in dph. Saturated and monounsaturated fatty acids decreased along larval development, while polyunsaturated fatty acid (PUFA) content increased. The ratio of docosahexaenoic acid (DHA) / eicosapentaenoic acid (EPA) shifted from 4 – 5 in early developmental stages to lower than 1 after metamorphosis. Results suggest a subdivision of the larval development into two stages of opposite FA requirements. <br> http://hdl.handle.net/2072/39469 title: Experimental exposure of the blue mussel (Mytilus edulis, L.) to the toxic dinoflagellate Alexandrium fundyense: Histopathology, immune responses, and recovery authors: Galimany Sanromà, Eva; Sunila, Inke; Hégaret, Hélène; Ramón, Montserrat; Wikfors, Gary H. <br>abstract: Mussels (Mytilus edulis) were exposed to cultures of the toxic dinoflagellate Alexandrium fundyense or the non-toxic alga Rhodomonas sp. to evaluate the effects of the harmful alga on the mussels and to study recovery after discontinuation of the A. fundyense exposure. Mussels were exposed for 9 days to the different algae and then all were fed Rhodomonas sp. for 6 more days. Samples of hemolymph for hemocyte analyses and tissues for histology were collected before the exposure and periodically during exposure and recovery periods. Mussels filtered and ingested both microalgal cultures, producing fecal pellets containing degraded, partially degraded, and intact cells of both algae. Mussels exposed to A. fundyense had an inflammatory response consisting of degranulation and diapedesis of hemocytes into the alimentary canal and, as the exposure continued, hemocyte migration into the connective tissue between the gonadal follicles. Evidence of lipid peroxidation, similar to the detoxification pathway described for various xenobiotics, was found; insoluble lipofuchsin granules formed (ceroidosis), and hemocytes carried the granules to the alimentary canal, thus eliminating putative dinoflagellate toxins in feces. As the number of circulating hemocytes in A. fundyense-exposed mussels became depleted, mussels were immunocompromised, and pathological changes followed, i.e., increased prevalences of ceroidosis and trematodes after 9 days of exposure. Moreover, the total number of pathological changes increased from the beginning of the exposure until the last day (day 9). After 6 days of the exposure, mussels in one of the three tanks exposed to A. fundyense mass spawned; these mussels showed more severe effects of the toxic algae than non-spawning mussels exposed to A. fundyense. No significant differences were found between the two treatments during the recovery period, indicating rapid homeostatic processes in tissues and circulating hemocytes. <br> http://hdl.handle.net/2072/39468 title: Pathology and immune response of the blue mussel (Mytilus edulis L.) after an exposure to the harmful dinoflagellate Prorocentrum minimum authors: Galimany Sanromà, Eva; Sunila, Inke; Hégarer, Hélene; Ramon, Montserrat; Wikfors, Gary H. <br>abstract: The harmful dinoflagellate Prorocentrum minimum has different effects upon various species of grazing bivalves, and these effects also vary with life-history stage. Possible effects of this dinoflagellate upon mussels have not been reported; therefore, experiments exposing adult blue mussels, Mytilus edulis, to P. minimum were conducted. Mussels were exposed to cultures of toxic P. minimum or benign Rhodomonas sp. in glass aquaria. After a short period of acclimation, samples were collected on day 0 (before the exposure) and after 3, 6, and 9 days of continuous-exposure experiment. Hemolymph was extracted for flow-cytometric analyses of hemocyte, immune-response functions, and soft tissues were excised for histopathology. Mussels responded to P. minimum exposure with diapedesis of hemocytes into the intestine, presumably to isolate P. minimum cells within the gut, thereby minimizing damage to other tissues. This immune response appeared to have been sustained throughout the 9-day exposure period, as circulating hemocytes retained hematological and functional properties. Bacteria proliferated in the intestines of the P. minimum-exposed mussels. Hemocytes within the intestine appeared to be either overwhelmed by the large number of bacteria or fully occupied in the encapsulating response to P. minimum cells; when hemocytes reached the intestine lumina, they underwent apoptosis and bacterial degradation. This experiment demonstrated that M. edulis is affected by ingestion of toxic P. minimum; however, the specific responses observed in the blue mussel differed from those reported for other bivalve species. This finding highlights the need to study effects of HABs on different bivalve species, rather than inferring that results from one species reflect the exposure responses of all bivalves. <br>